Creation, Breeding, Phenotyping, Distribution and Archiving of model organisms

Phenotyping tools

Behavior, Cognition & sensory organs

Several protocols adapted to rodents (Phenomin-ICS, Chronobiotron, Anexplo) or non-human primates (MPRC) are available for evaluation of a wide range of central and peripheral nervous system functions or their pathologies. Tests may be used as a packages for phenotyping genetically modified mice and rats, to study environmental disease models or to screen for potential therapeutics. Alternatively, a panel tests may be customized with our expert consultancy for rapid and targeted discovery.

 

Openfield
Light-dark
Elevated plus maze
Tail suspension
Forced swim
Sucrose preference
Acoustic startle and prepulse inhibition
Cross-modal prepulse inhibition
Amphetamine-stimulated locomotor activity
Social recognition
Social interaction
Actimetric cages
Clinical observation by modified SHIRPA
Grip strength
Rotarod
String test
Automated gait analysis
Balance beam
Notched beam
Mouse reaching and grasping
One meter swim test
Tail flick
Hot plate
Formalin
Von Frey test
Hargreaves
Electromyography (EMG)
Electroencephalography (EEG)
Optomotor response
Slit lamp
Eye fundus imaging
Optical coherence tomography
Angiography (fluorescent imaging)
Electroretinography
Auditory brainstem response
Active avoidance
Associative memory (pavlovian fear conditioning)
Shock threshold
Morris water maze
Novel object recognition
Object location memory
Pentylene-tetrazol (PTZ)-induced seizures
Intellicages
Touchscreen
Y-maze spontaneous alternation 
Stereotactic surgical procedure for rodents and primates
Pharmacological treatments

Cardiovascular exploration

Non invasive blood pressure
Electrocardiography
Echocardiography
Telemetry
Vascular exploration
Heart and vessels histological examination
Anatomopathology

Metabolism

Growth rate, body weight progression
Body composition
Body temperature measurement
Food and water intake
Energy expenditure/Indirect calorimetry
Calorimeter bomb
Glucose tolerance test (GTT)
Insulin sensitivity test (IST)
Euglycemic clamp
Skeleton X-Ray analysis
Bone mineral density
Bone mineral architecture
Muscle contractility
Muscle force
Exercise test
Metabolic and diuresis cages                  
Urine analysis
Cold challenge
High fat diet challenge
Restriction diet
Sodium diet

Immunology Inflammation, Infection & vaccination

Flow Cytometry multiparametric analysis of the immune system cell types
Mass Cytometry multiparametric analysis of the immune system cell types
BSL3 infection profiling
Real-time imaging in BSL3 conditions
Vaccine assay in BSL3 conditions
Imaging of radiolabeled macrophages, granulocytes phagocytosis and recruitment by lesions
Monoclonal antibodies production (TRIP)
16S metagenomic gut microbiota analysis
Anatomopathology
Blood chemistry

Clinical Chemistry, Haematology & Homeostasis

Common plasma chemistry
MultiPlex endocrine panels
Elisa/RIA endocrine analysis
Complete blood count
Coagulation
Metabolic and diuresis cages
Urine analysis
Immunoglobulins
Cytokines
Facs analysis
Biological analyses for NHP (SdP??)
Neurochemistry (AniRA)

Lung function exploration

Spontaneous breathing
Airway responsiveness
Bronchoalveolar lavage fluid
Ovalbumin-induced asthma model
HDM-induced lung inflammation
LPS-induced lung inflammation
Lung imaging by microCT
Nephrectomy

Oncology

Tumor cells injection
Echo-guided injection
Immunoprofiling
Tumor analysis by spectral cytometry (up to 5 cell populations and activation markers) on tumor
BSL2 and BSL3, equipped with imaging (bioluminescence/ fluorescence)
Ultrasound imaging of tumors
X-Ray imaging
Human & cancer cells biomarkers (DNA, mRNA)
Clinical blood chemistry
Anatomopathology

 

Anatomopathology & Imaging

Several techniques and tools for tissue preparation, staining or visualization are used in Tefor Paris Saclay, Phenomin centers, Anexplo and MPRC for evaluation of structural and pathological hallmarks of diseases in different species.

  

Necropsy
Histopathological evaluation of organs
Fixation
Tissue processing, embedding, sectioning
Staining
Immunostaining
In-situ hybridization
Cell proliferation
Apoptosis
Wound Healing
Pattern of β-galactosidase expression
Lethality window determination
Embryonic and perinatal defects
Gene expression pattern
Slide scanning for high through put histological analysis
Tissue Clearing in big specimens
Fast 3D-screen for toxicology
High reactive index matching
Confocal and multiphoton microscopy
High-Resolution Episcopic Microscopy (HREM)
Non-invasive imaging tools for small animals: CCD cameras (bioluminescence/ fluorescence), nanoSpect/CT, microTEP/CT (Phenomin, Anexplo)
X ray imaging (microCT, Computed X Ray Tomography, Dual X-RayEmission analysis (DEXA))
Multi-scale in vivo imaging for Non human Primates

Ultrasound imaging (ECHO)

Electrophysiology

Electroencephalography (Phenomin-ICS, MPRC; Chronobiotron)
Electrocardiography (Phenomin-ICS, Chronobiotron)
Electromyography (Phenomin-ICS, MPRC, Chronobiotron)
Electroretinography (Phenomin-ICS)
Auditory brainstem response (ABR) (Phenomin-ICS)

Surgery & Pharmacology

Most of Celphedia centers have specialized staff and equipments to perform a wide range of surgical procedures and pharmlacological studies.

Microsurgery for rodents (Anexplo, Phenomin-ICS, Chronobiotron)
stereotaxic surgery for rodents (Phenomin-ICS, Chronobiotron)
Surgery and steretotacxic surgery for non-human primates (MPRC)
Microdialysis (Chronobiotron)
Systemic injection routes in anesthesized or freely moving animals (intraperitoneal, per os, subcutaneous, intravenous,...)
brain injection in anesthesized and freely moving animals
Preclinical trials for all species