TEFOR-AMAGEN (UMS 3504 CNRS / UMS 1374 INRA) is located on 2 sites: Gif-sur-Yvette and Jouy-en-Josas. Its aim is to promote the utilization of aquatic animal models. TEFOR-AMAGEN has developed two models of teleost fishes: medaka (Oryzias latipes) and zebrafish (Danio rerio). The issues of modern biology involve the implementation of techniques allowing comparative analysis of living organisms.
Among vertebrates, the fish model displays easy high density breeding (short generation time), favourable biological peculiarities (accessibility and transparency of the eggs, genome sequenced for both species used on the platform), original technological approaches (confocal and two-photon microscopy), as well as a wide accessibility to fully transparent larvae usable up to 5 days after fertilization.
Transgenesis is an essential technique for studies in vivo. In a research and development process improving the transgenesis techniques in both species, TEFOR-AMAGEN will provide customized transgenic lines to European research teams.
Since its creation in 2009, TEFOR-AMAGEN has conducted research projects in the area of high specificity nucleases to develop targeted transgenesis and genome editing methods. In the frame of TEFOR, an infrastructure laureate of Future Investments in 2012 and focusing on non-mammalian animal models, TEFOR-AMAGEN has increased its capacity to produce zebrafish lines. The platform now proposes techniques to generate zebrafish lines via genome editing and KO (Knock-Out) models.
Aquatic animal models
- To develop and promote the utilization of aquatic animal models by scientific researchers : two models of teleost fishes (medaka, Oryzias latipes and zebrafish, Danio rerio).
- To improve breeding techniques of these aquatic species and to make the results available to the international community.
- To maintain an in-depth expertise in the transgenesis of aquatic species
- To develop and implement new techniques of:
• transgenesis in aquatic species
• characterization and detection of transgenes in aquatic species
- To develop efficient transgenesis vectors available to the scientific community by using the latest advances in that field
- To help research units to implement medium and high throughput phenotyping protocols.
Specific trainings dedicated to aquatic species
- Regulatory training for people developing experimental procedures on "aquatic species"
A 57-hour course (45 hours training courses + 12 hours practical work) for research scientists and engineers managing a direct scientific authority in experimentation on animals. This training course includes the program for staff participating to experimentation on animals, such as technicians. That multi-site CNRS training has been implemented thanks to the collaboration of three partner centers of CELPHEDIA: TEFOR-AMAGEN, CRB Xenopus and CE2F-PRIM.
Training content specific to aquatic species:
① Regulation in the use of animals on scientific purposes
② Transportation of aquatic animals
③ Ethics: review of ethics procedures, ethics committees, the 3R rule, pain scale and assessment of severity, animal welfare and stress, analgesia, pain and nociception, euthanasia
④ Which animal for what model? Aquatic models.
⑤ Handling of animals, anaesthesia process, pre- and postoperative care
⑥ Zoology, comparative anatomy, general physiology, ethology, reproduction
⑦ Management of environmental parameters, management of an aquatic animal facility
⑧ Infectious diseases and health status, diseases of aquatic animals bred in captivity
Trainings exclusively limited to CNRS employees or non-CNRS employees working in a CNRS unit: 7 days
▪ 2 sessions in Marseille in 2018: April 5-13, September 13-21
- Breeding, behaviour and transgenesis of fish models: zebrafish and medaka
Aims of the training:
① To know the rules required to start a farm of fish-models
② To acquire the theoretical bases of fish transgenesis
③ To understand the basics of biology concerning Danio rerio et Oryzias latipes species
④ To achieve experiments involving injections into medaka or zebrafish eggs: transgenesis, morpholino injection, mRNA injection, …
⑤ How to use zebrafish models in behavioural experiments
Training organized by "CNRS formation entreprises": 4 days
▪ 2 sessions in Gif-sur-Yvette in 2018: March 27-30, November 6-9
- Management of a fish models facility (zebrafish and medaka)
Immersion training for one single trainee to get an overview of the management of an aquatic animal facility.
▪ Immersion training in Gif-sur-Yvette (3 days)
- Injection and transgenesis of fish models (zebrafish and medaka)
Immersion training for one single trainee
▪ Immersion training in Gif-sur-Yvette (2 days)
- Regulatory training for staff developing experimental procedures on aquatic species
① To be in agreement with animal experimentation regulation (French decree of February 1st ,2013)
② To be trained to use laboratory animals compliant with animal welfare
③ To be graduated in animal experimentation
▪ Nantes in 2018 (7 days): June 12-20 at ONIRIS, Veterinary School of Nantes
- Courses on aquatic species for trainings on animal experimentation at the Lycée d'Enseignement Général et Technologique Agricole de Vendôme.
TEFOR-AMAGEN is involved in several ambitious projects connecting academic laboratories and private companies
TEFOR-AMAGEN has developed a transgenesis platform entirely dedicated to the production of models for scientific research, either public or private, French or European.
- Development of Knock out (KO) technologies targeted via TALE nucleases, CRISPR and meganucleases
- Development of transgenesis and FO phenotyping via TALE nucleases (in the frame of TEFOR infrastructure)
- A set-up project of a zebrafish animal facility displaying a high health status level: SPF (Specific Pathogen Free/EOPS).
- In the frame of its involvement in TEFOR infrastructure and in cooperation with their imaging service as well as with academic partners specialized in zebrafish phenotyping, TEFOR-AMAGEN contributes to the development of new phenotyping tools in confocal imaging of the whole body with cellular resolution. The aim is to provide the scientific community with the required tools to perform expression profile screening of regulatory sequences directing transcription and to promote 3D imaging of transgenic lines.