Centre de Formation et d’Exploration Fonctionnelle PRIMates (CE2F-PRIM)

The CE2F-PRIM (Centre de Formation et d'Exploration Fonctionnelle sur modèle PRIMates non humains) (UMS 3537-CNRS) is a unique structure in France and Europe for its functional exploration work in non-human primates (NHP), notably in neuroscience, and for its training activities in the use of animals for scientific purposes.

The centre offers accommodation capacities for non-human primates (Macaca mulatta, Macaca fascicularis, Saimiri sciureus) in outdoor parks or indoor housing, as well as various rodent species, for basic and clinical neuroscience experiments. The centre has A1/A2 housing areas and several ultra-modern surgical platforms dedicated to animal research in NHP and rodents.

Among its scientific and functional objectives, the CE2F-PRIM wishes to set up a structure for the production of neurological pathologies animal models using conventional and molecular approaches, in response to the limitations of mouse models. In close collaboration with the Institut de Neurosciences de la Timone (UMR7289), but also with the Station of Primatology (SdP) in Rousset, CE2F-PRIM is setting up a complete chain for the use of the NHP model in biomedical research, in response to the limitations of mouse models, ranging from resource management to multimodal functional exploration (electrophysiology, multi-scale in vivo imaging). The Centre supports basic research, clinical or private (Biotechnologies, CRO...).

With more than 30 weeks of training per year, including all regulatory training, thematic trainings on different animal models and on surgical models, CE2F-PRIM is one of the most important animal regulatory training centres in Europe.

CE2F-PRIM participates with the SdP to the activities of EUPRIM-Net II Consortium. This network gathers nine European primatology centers whose aim is to combine their extensive biological and biomedical research activities, in-depth knowledge, infrastructure resources and solid experience in hosting primates.

CE2F-PRIM is IBiSA certified.

The CE2F-PRIM was created to fulfil 3 main missions. The first objective is to support neuroscience research within INT (Institut de Neurosciences de la Timone) and other laboratoriesin Marseille, particularly for the production of animal models. The second mission is the training of research staff. Its third mission is to provide services (housing, follow-up of biological protocols, experimental surgery) for biomedical R&D of private partners in different fields: neurosciences, pharmacology and immunology.

Support for research

In conjunction with other INT technological platforms, CE2F-PRIM provides access to functional in vivo imaging tools, particularly to conduct experimental protocols. CE2F-PRIM upgrades experimental devices used for animals to improve functional and behavioural exploration devices while improving animal welfare.

  • INT support activities mostly focus on functional exploration in non-human primates:
    ◊ Neurosurgical operations
    ◊ Invasive and non-invasive techniques to decode brain activity and its relationship to behaviour
    ◊ Mass electrophysiology
    ◊ Molecular imaging (PET/SPECT), microscopic (confocal, biphotonic), mesoscopic (optical), macroscopic (3T MRI)
    ◊ Recording studies of motor behaviour and perceptual function disturbances

The CE2F-PRIM ensures all the essential steps to work with animals in optimal conditions of safety, animal welfare, veterinary care and ethics. Different animal models are available (rats, mice, non-human primates).

Trainings

In terms of training, CE2F-PRIM is a national reference center for CNRS et l’INSERM.

  • Each year, CE2F-PRIM organizes more than 30 weeks of regulatory training at all levels (Animal Experimentation "operator" and "designer" levels, Surgery, Zootechnics) for the use of animals for scientific purposes (rodents, non-human primates, pigs, aquatic vertebrates).
    These regulatory training courses are organized in Marseille, but also in Paris area, Bordeaux, Strasbourg, and are intended for research staff: animal technicians, researchers and students.
  • CE2F-PRIM also hosts specialized trainings (electrophysiology, experimental ophthalmology,...) in partnership with the Marseille Faculty of Medicine and private partners.

Value creation

  • Services for biotechnology companies and academic units wishing to conduct their R&D projects in non-human primates or rodents.
  • Support to academic research teams through its surgical platform and accommodation structures

CE2F-PRIM organizes more than 30 weeks of training sessions for scientific and technical staff, either academic or private.

CE2F-RPIM organizes regulatory training in animal experimentation, conform to the requirements of the new European Directive 2010/63 (former levels I and II, and surgery).

The objective is to acquire the mandatory qualification to practice animal experimentation in accordance with the transposition of the new European Directive 2010/63.

CE2F-PRIM also organizes specific trainings in primaology and neurosciences.

Regulatory trainings for designing animal experimental procedures (former level 1)

Regulatory training for researchers and research engineers with direct scientific responsibility for animal experimentation, designing or carrying out experimental procedures.
The teaching covers animal experimentation regulations and legislation (national regulations and new European directive) as well as experimental procedure design, mutagenesis, ethical principles and applied ethics, the 3Rs rule, alternative methods, basic knowledge of model animal biology, zootechnics, animal health status, animal pain and stress, pathologies, anaesthesia techniques, euthanasia, autopsy,...

Designing experimental procedures on rodents

  • CFE trainings "CNRS formation entreprises": 7 days (no e-learning)
    ▪ 2 sessions in Gif-sur-Yvette in 2019: May 20-28, November 20-28
    ▪ 2 sessions in Gif-sur-Yvette in 2020: May 11-19, November 16-24

    Description
  • Trainings exclusively limited to CNRS employees or non-CNRS employees working in a CNRS unit: 5 days + e-learning
    ▪ 4 sessions in Paris en 2019: March 11-15 with e-learning from 28/01/19 to 01/03/19, April 15-19 with e-learning from 04/03/19 to 05/04/19, September 9-13 with e-larning from 01/07/19 to 31/08/19, October 21-25 with e-learning from 09/09/19 to 11/10/19
    ▪ 3 sessions in Marseille in 2019:
    February 11-15 with e-learning from 02/01/19 to 01/02/19, March 25-29 with e-learning from 11/02/19 to 15/03/19, September 30 to October 4 with e-learning from 01/07/19 to 20/09/19
    ▪ 1 session in Bordeaux in 2019:
    June 17-21 with e-learning from 06/05/19 to 07/06/19

    Registration procedures

  • Regulatory training session in ENGLISH for staff developing experimental procedures and projects on "rodents" organized by CE2F-PRIM and CELPHEDIA
    Training limited to non-Francophone students and researchers working in a CNRS unit: 7 days
    ▪ 1 session in Marseille in 2019: July 1-9
    ▪ 1 session in Strasbourg in 2019: October 10-18

    Registration procedures

Designing experimental procedures on non human primates

  • Regulatory training for people designing procedures on non human primates
    This regulatory training is intended for people designing experimental procedures and projects on non-human primate (NHP) models.
    This teaching also discusses the relevance of using the NHP model for scientific purposes:
    ① Understand in which research themes the NHP model remains unavoidable and why?
    ② Discover and better understand the possibilities of medical imaging applied to the NHP model
    ③Demonstrate the importance of experimental models other than the NHP model
    ④ Reuse of animals in research
    Trainings exclusively limited to CNRS employees or non-CNRS employees working in a CNRS unit: 7 days
    ▪ Session in Marseille in 2019: March 7-15

    Registration procedures

Designing experimental procedures on aquatic vertebrates

  • Regulatory training for people designing procedures on aquatic models
    This regulatory training is intended for research scientists and engineers managing a direct scientific authority in experimentation on aquatic models. CNRS training set up by collaboration of three partner centers of CELPHEDIA: TEFOR-AMAGEN, CRB Xenopus and CE2F-PRIM.
    This specific training for aquatic species covers the regulation, transport of aquatic animals, ethical issues, pain and severity assessment, euthanasia methods, animal handling, anatomy, reproduction, management of environmental parameters (water), operation of an aquaculture animal facility, infectiology and health status.
    CFE trainings "CNRS formation entreprises": 7 days
    ▪ 2 sessions in Marseille in 2019: June 17-25, October 21-29
    ▪ 2 sessions in Marseille in 2020: June 15-23, October 19-27
    Description
    Trainings exclusively limited to CNRS employees or non-CNRS employees working in a CNRS unit: 7 days
    ▪ 3 sessions in Marseille in 2019: January 21-29, May 16-24, September 12-20
    Registration procedures

Regulatory trainings for application of animal experimental procedures (former level 2)

Regulatory training for personnel applying experimental procedures on animals.
The teaching covers animal experimentation regulations and legislation as well as the animal (ethics, 3Rs rule, design of experimental procedures, zootechnical procedures, housing, genetics, basic knowledge of rodent biology, small animal imaging, pathologies, anaesthesia, analgesia and euthanasia, use of appropriate limit points, recognition of signs of distress and pain, hygiene and health control, transport).

Applying experimental procedures on rodents

  • CFE trainings "CNRS formation entreprises": 5 days (no e-learning)
    ▪ 4 sessions in Gif-sur-Yvette in 2019: April 8-12, June 3-7, September 16-20, October 7-11
    ▪ 3 sessions in Gif-sur-Yvette in 2020: March 30 to April 3, June 8-12, October 12-16
    Description
    ▪ 4 sessions in Marseille in 2019: March 18-21, May 13-16, October 14-17, December 2-5
    Description

  • Trainings exclusively limited to CNRS employees or non-CNRS employees working in a CNRS unit: 5 days + e-learning
    ▪ 1 session in Marseille in 2019: April 8-12 with e-learning from 25/02/19 to 29/03/19

    Registration procedures

Regulatory trainings in surgery

Regulatory training in surgery limited to people having already attended regulatory trainings either for designing or for applying experimental procedures and requiring authorisation to perform surgical procedures on animals, in accordance with the transposition of the new European Directive 2010/63/EU.
The teaching covers surgical techniques and preparatory and post-operative care: asepsis, preparation of animals and staff, organization of the premises, choice and maintenance of equipment, anaesthesia, analgesia monitoring, postoperative follow-up, limits in surgery, infection prevention, microsurgical techniques.

  • CFE trainings "CNRS formation entreprises": 4 days
    ▪ 4 sessions in Marseille in 2019: March 18-21, May 13-16, October 14-17, December 2-5
    ▪ 3 sessions in Marseille in 2020:
    March 16-19, May 11-14, October 12-15

    Description

  • Trainings exclusively limited to CNRS employees or non-CNRS employees working in a CNRS unit: 4 days
    ▪ 5 sessions in Marseille in 2019: February 4-7, April 1-4, June 3-6, September 23-26, November 18-21
    Registration procedures

Ethics

  • The 3 Rs in practice, refinement, endpoints
    This training, which is intended for designers, practitioners and keepers in animal experimentation, entitles them to 3.5 hours of training to maintain their skills (decree n°2013-118).
    Objectives:
    ① Update your knowledge of animal experimentation
    ② Validate your practices in compliance with the regulations relating to decree n°2013-118
    ③ Be aware of the endpoints to optimize their implementation and application in experimental procedures
    Training "CNRS formation entreprises": half a day
    ▪ Session in Orleans in 2020: March the 3rd
    Description

Trainings in neurosciences

  • Surgery training on rodent model
    Training organized in collaboration with NSRepair company
    This training covers regulatory practices in surgery and ethics, preparation of an intervention (instruments, animals) and surgical approaches to the brain, cerebrovascular system, spinal cord, peripheral nervous system.
    Applications to models of stroke, ischemia, spinal cord injury or peripheral nerve injury.
    ▪ Session in Marseille: next dates not yet defined
  • European Surgical Training for the Utah Electrode Array
    Training on multi-électrode implants organized in collaboration with the US company Blackrock Microsystems.
    The purpose of this workshop is to familiarize participants with the skills required for effective surgical implantation of Utah Array™ in non-human primates.
    ▪ Session in Marseille: next dates not yet defined

Trainings for zootechnicians

  • Participation to the Network Days of the CNRS Zootechnicians (ReZooTech)
    The programme focuses on regulatory developments with the new EU Directive 2010/63/EU and its implementation.
    Trainings exclusively limited to CNRS zootechnicians
    Next session: date and place to be defined

Other trainings

Modules from ½ or 1 day of continuing practical training (sampling methods, anesthesia,...)

Neurosciences

  • Implementation of a structure for the production of neurological pathologies animal models using conventional and molecular approaches, in response to the limitations of mouse models.
  • Functional exploration in neurosurgery on non-human primate and rodent models, to decode brain activity and its links with behaviour
  • Production of PNH models of neurological and psychiatric pathologies

Imaging

  • Development of a biphotonic microscopy system in non-human primates
  • Development of anatomical and functional imaging by 3T MRI on the non-human primate model

CE2F-PRIM logoMarseille

CE2F-PRIM logoNon-human primates, rodents

CE2F-PRIM logodata not available

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